Both microplate and cuvette ports can be qualified. Total phenolic contents were calculated from the gallic acid standard curve. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 4. Operated fromTo ensure loading of equal amounts of protein for each sample, colorimetric protein quantification was performed using a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA) and a SpectraMax M3 spectrophotometer (Molecular Devices, Sunnyvale, CA). SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. The SpectraMax M2 and M2e are versatile and reliable. 1. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. 4. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Multimode Reader Comparison Chart. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. SpectraMax Luminometer Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Italic and boldface type are used for emphasis. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. reader in the visible range between 405–750 nm with NIST and NMI. The SpectraTest ABS1 Validation Plate package. 2. 1 Defect ID: FB 2570 These two validation protocols are not included in the v5. 1. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. 314 low lamp level warning. Wavelength Range. 0200-0729. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. 4. Mitochondrial ROS concentration was determined and compared with H 2 O 2 standards using a 96-well black plate and a spectrofluorometer (SpectraMax ® M3; Molecular Devices, San Jose, CA, United States) at 540-nm emission and 580-nm extinction (Maciel et al. The SpectraMax M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for. Description 1. As for each measurement, I. DevicesThe SpectraMax M2 And M2e Systems Provide The Multi Detection Microplate Readers With Dualmonochromators, Dual-mode Cuvette Ports, With Top & Bottom Reading. Page 26 The trademarks used herein are the. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Accuris SmartDrop. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Generate more data points without the need to dilute. The resulting absorbance values were plotted against time. spr SpectraMax Plus 384 SpectraTest ABS2 v1. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization Options in the instrument settings in SoftMax Pro Software. Our research provides a. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The innovative and flexible design features. Molecular Devices LLC pro 6 3 software controlled spectramax m3 multimode microplate reader Pro 6 3 Software Controlled Spectramax M3 Multimode Microplate Reader, supplied by Molecular Devices LLC, used in various techniques. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. book Page 1 Wednesday, July 7, 2010 2:09 PMA SpectraMax ® M3 microplate reader luminometer was used to quantify the chemiluminescence from the reactions as relative luminescence units (RLUs) every four seconds for three minutes. 3 software. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. 0112-0112F 3 Contents Chapter1:SpectraTestABS1ValidationPlateOverview 5 CertificateofCalibration 6 ISO-17025 6 AvailableTests 7 Chapter2:ValidationProtocols 9 SpectraMax® L (software version 5. The luminescent signal was proportional to the amount of GSH or GSSG present. Absorbance was read at 570 nm using a SpectraMax M3. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. We measured the fluorescence intensity for 240 min at room temperature using a spectrophotometer (SpectraMax M3, Molecular devices) with excitation and emission wavelengths of 485 and 525 nm, respectively. 7. Multi-Mode Microplate Readers. Fluorescence was quantified on SpectraMax M3 plate reader, Ex 485/Em 520 using SoftMax Pro software. Loosen the captive screws on the front of the instrument and then raise the top cover up and over the instrument. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. . U2OS cell viability (%) = [A cells with different treatments −A contro ]/[A cells with no treatments − A control ] × 100. The cuvette must be in place when you read the plate. Description1 Introduction The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. When the user applies a set temperature of 37°C, the top is heated to 37. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SpectraTest ABS1 Absorbance Validation Plate. RP-3500 is highly selective for ATR with 30-fold selectivity over mammalian target of rapamycin (mTOR) and more than 2,000-fold selectivity over ataxia. Fusion of liposomes was followed by content mixing of the RPLs and subsequent increase in fluorescence which was monitored in a SpectraMax M3 fluorescence plate reader (Molecular devices). 21. Examples: “Press carefully to engage,” “ Do not. After cooling to room temperature (22 °C), the mixture was transferred to 96-well plates. The bacterial colony was mixed with. In the M2, M5 and Plus 384, the light. The reaction was terminated with 100 µL of dinitrosalicylic acid and placed in a boiling water bath for 5 min. Also refer to this Multimode Reader Comparison Chart. , 2020). To measure cell viability, 74 µl/well CyQUANT ® NF DNA binding dye was added to the cells and the cells were incubated for 60 min at 37 °C before fluorescence was measured using 485 nm excitation and 530 nm emission using a Spectramax M3 multi-mode microplate reader. ROS detection. 01) + 0. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. Choose from a three- (M3), four- (M4), orThe SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 2. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. book Page 1 Wednesday, July 7, 2010 2:09 PM FlexStation® 3 Benchtop Multi-Mode Microplate Reader User Guide 0112-0127 B June 2013 FlexStation_manual. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Find regional support. SpectraMax® Paradigm® Multi-Mode Detection Platform. 1). For more information, please visit us at per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. We would like to show you a description here but the site won’t allow us. 1. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Luminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. When a SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. . 4. SpectraMax® M5e. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. The corrected absorbance is within 5% of the. Fluorescence Microplate Reader Comparison Chart. 1 & 7. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The supernatant was collected after 20 min of incubation in the dark. HNE (EC 3. Qualify the fluorescence performance of the SpectraMax . Choose from a three- (M3), four- (M4), orFluorescence was measured using a SpectraMax M3 plate reader equipped with an excitation at 490 nm and an emission at 535 nm. Bottom-reading is usually recommended for cell-based assay with adherent cells. Bioz Stars score: 86/100, based on 1 PubMed citations. 1. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. The drawing shows a standard microplate of 15 mm height covered with a lid. Expand your lab’s capabilities to include flash applications such as dual luciferase and. Moffitt Cancer Center. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Extramitochondrial ROS concentration was determined and compared with H 2 O 2 standards using a 96-well black plate and a spectrofluorometer SpectraMax® M3 at 540 nm emission and 580 nm. 10. Polyplex sizes and zeta potentials were measured with a Malvern Instruments Zetasizer (Nano-2590) at a scattering angle of 173°. Polyplex Size and Zeta Potential Measurement. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Microtitre plates were incubated for 2 h at 37°C in the dark. 3) At the bottom of the strip, click on "Show. SpectraMax M3, M4, M5, and M5 e. . Multimode Reader Comparison Chart. Bioz Stars score: 96/100, based on 1 PubMed citations. The plate was placed into the SpectraMax L Luminometer and a 1-second integrated read taken of each selected well using the MycoAlert protocol in the SoftMax ® Pro Software (Read A). Detection modalities are shown in Table 1-1. When a feature or capability applies to only certain readers, this exception is noted. SpectraMax® ABS Plus. 4 kilograms). Data were expressed as mg/g gallic acid equivalents (GAE) of dry crude extract. Description 1. For more information, please visit us at using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. Page 28 Chapter 5: Read Modes and Read Types 1. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. User Manual – SpectraMax M3 M4 M5 M5e. We would like to show you a description here but the site won’t allow us. Description 1. Published Dec 16, 2020 | Updated Dec 16, 2020. Category 2 and 3 microplate readers use gradient heating as well. com. SpectraMax Microplate Readers. BioTek Synergy 2 SL Luminescence Microplate Reader with PC & Gen5 Software. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. For wavelengths in the UV range above 220 nm,. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Table 1. For a complete list of the instruments supported by this release of the8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. edu, 814-863-3503. All the experiments were done in triplicate. Bioz Stars score: 96/100, based on 1 PubMed citations. Sale. 6 (rated by 49 users) Compatible devices: FilterMax F3, SpectraMax MiniMax 300, SkanWasher 300, Digidata 1550, ClonePix 2, SpectraMax iD3, SpectraMax Paradigm, SpectraMax. 100 µL of MycoAlert Substrate (reconstituted in MycoAlert Buffer) was immediately added to each well and the plate incubated at room temperature for 10 minutes. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 50 0112-0115G Item Description Wavelengthrange 200-1000nm Wavelengthselection Monochromator,tunablein1nmincrementsPage 25 SpectraMax iD3 Multi-Mode Microplate Reader Installation Guide To install the transport lock on the plate drawer: 1. 1step assay with SpectraMax microplate readers. SpectraMax Line of Readers Now Includes 14 Unique Configurations to Accommodate Wide Range of Customer Detection Needs MDS Analytical Technologies Launches Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate ReadersAutomated Functions Autofocus, autoLED intensity, auto-exposure Autofocus Method Image-based autofocus User-trained autofocus Laser autofocus (option)2. SpectraMax Paradigm Multi-Mode Detection Platform User Guide Loading and Unloading Microplates To load or unload a microplate: 1. DCFH-DA and JC-1 were used to detect intracellular ROS and mitochondrial membrane potential. 1. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® iD3 Multi Mode Microplate . 0 out of 5 $ 5,999. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Dual monochromators increase performance and assay flexibility. Other technologies such as imaging. After 20 min incubation at 37 °C in the dark, the plates were analyzed by a SpectraMax M3 microplate reader to measure the absorbance at 405 nm. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The SpectraMax Multi-Mode Microplate Reader offers both the Cuvette. We would like to show you a description here but the site won’t allow us. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forIntroduction. The DMAC reagent was replaced by EtOH in the control wells. 00 . "With this complete portfolio of multi-mode configurations, we can deliver multi-mode. This version of Molecular Devices SpectraMax M3 Manual compatible with such list of devices, as: SpectraMax M3, SpectraMax M4, SpectraMax M5,. Cables. EarlyTox Live Cell Assay (6. Category 2 and 3 microplate readers use gradient heating as well. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10 The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Features. In the SoftMax Pro Software, select the Operation tab and click Change Aperture to display a message. SoftMax Pro GxP is a special software release that includes full user and permission con-SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and. The spectrophotometer (SpectraMax, M3, Molecular Devices, Washington DC, USA) was used to confirm the encapsulation of gallic acid inside the PLGA nanoparticles. 1 release preventing the protocols from being used for validation. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. NETosis Assays Using Immunostaining and Microscopy. When the user applies a set temperature of 37°C, the top is heated to 37. Using the PathCheck® Sensor, results can be automatically normalized to a 1 cm pathlength so that the values are. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. 313 reference gain check fail. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Serum IL-8 level quantification Serum IL-8 levels were quantified in sera from patients after HSCT using an enzyme-linked immunosorbent assay (Cat. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. For more sample throughput, the system can StakMax Handling Stacker Base Unit. Our Price: $8,635. 00 . The relative number of viable cells was quantified by measuring the fluorescence (excitation at 560 nm and emission at 590 nm) using a SpectraMax ® M3 (Molecular Devices, San Jose, CA, USA). Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The following document is intended to demonstrate setup of this instrument. The concentration of CTC was expressed as mg catechin equivalent per g of extract. Use the 2. Fluorescent Polarization: Solid black plates, as above. In addition, the total flavonoid content was determined using the aluminum chloride method. spr SpectraMax iD3 SpectraTest ABS2 v1. The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. Place the microplate on or remove it from the microplate drawer. • Tel. Molecular Devices Spectramax Plate Reader. The reading was taken with a spectrophotometer (SpectraMax M3) at 640 nm. Abstract. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 1. Multimode Reader Comparison Chart. 100 readings per well gives optimal Z’ values for this assay. The SpectraMax M3 microplate reader (Molecular Devices, USA) was used to monitor the colour development over 5-min intervals for 30 min at 370 nm. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. WARNING! The instrument weighs approximately 36 pounds (16. . . The SpectraMax® M4 Multi-Mode Microplate Reader dual monochromator optics allow the widest range of applications without the need to change filters and delivers single-mode performance in one multi-mode platform. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. For standard curve values, an albumin standard (Thermo Fisher Scientific) dilution serious. Bioz Stars score: 86/100, based on 1 PubMed citations. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Data were collected using SoftMax Pro 6. Note: you might need to slide the microplate reader on the foam pad to make room for the cytometer. SpectraMax Mini. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. SpectraMax Glo and DuoLuc. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Ataxia telangiectasia and Rad3-related (ATR) kinase protects genome integrity during DNA replication. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 4. Detection modalities are shown in Table 1-1. M5 reader microplate m3 spectramax molecular devices llc m5e biocompare quote fluorescenceMolecular devices spectramax plus 384 user manual pdf download Spectramax® m2/m2e multimode microplate readers, with softmax® proMolecular devices spectramax m5 microplate reader labx contract svc warranty available manufacturer. Features. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. D8000C; R&D Systems, Minneapolis,. 8. no. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Capture Flash Assays with Ease. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. Additionally, SoftMax® Pro Software automatically identifies the optimal excitation and emission wavelengths via the Spectral Optimization Wizard. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax M 系列多功能微孔板读板机（酶标仪）可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. e. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. Multi-Mode Microplate Readers. Please contact Tatiana to reserve the plate reader time: tnl1@psu. 37) was ordered from Sigma Aldrich (St. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the barriers to novel, landmark discoveries. Antiretroviral activities. 2 release. The intracellular ROS level was also cross-confirmed using the DCF-DA method with a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA,. SpectraMax® ABS. Both readers feature an 8-channel read head for fast reads and. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. Mitosox works fine in 96 wells. MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. Concisely, digested test composite was treated with NaNO 2 (5%) and incubated for 5 min. Speed Read Mode. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. Expanded wavelength coverage. Multimode Reader Comparison Chart. Page 1 SpectraMax ® GEMINI EM Dual-Scanning Microplate Spectrofluorometer Operator’s Manual 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0088 Rev. a SpectraMax M3 microplate reader (Molecular Devices Inc. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Spectral Range. 1. Immunofluorescence ASC antibody (1:200, Abcam, ab155970, UK) was used for immunofluorescence in P. The isolates were categorized. 2 only) SpectraMax M-series Microplate readers. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Learn more about its features, specifications, and applications in this user. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics光验证板，用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 160 多种预存实验方法可以直接调用，可以进行数据获取和个性化的数据分析；强大的图标绘制工具和文本编辑功能，可以方便的进行各种数 据结果的展示。Popular answers (1) Sanjay Premi. Learn how to set up protocols, customize reports, export results, and more with this PDF document. Place the plate drawer transport lock on the end of the plate drawer. Figure 2-23: Bottle holder To install the bottle holder: 1. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. 306 plate air cal. com. The fluorescence of the reaction was read every 3 min (λ excitation = 485 nm, λ emission = 538 nm) for up to 90 min at constant 37 °C with the SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. Use the 3. Tested w/warranty. Example: [Drawer]. SpectraMax® M5. Subsequently, the PBS solutions containing the cells and the MSNs were incubated at 37°C under shaking at 220 rpm for another 4 h, after which the PBS supernatants were transferred into the wells of a 96-well plate and evaluated on a fluorescent spectrophotometer (SpectraMax M3, Molecular Devices, USA) using an. 0200-6117. Finally, SpectraMax M3 microplate reader was used to detect 412 nm absorption values, which were positively correlated with GSH levels. The team have compared the effects of antibiotics on bacterial viability and. The actual pathlength, d, of a solvent is found from the following equation: d cm. 2 software (Sunnyvale, CA, USA). 0 mm hex key to tighten screws #2 and #3 until the lock is attached to the plate drawer. SpectraMax i3 Multi-Mode Platform A flexible, user-upgradeable microplate detection system Key Features The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. 9000-0756. 315 can't find zero order. SpectraMax® iD5. The result was expressed as mg gallic acid. S. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Add cells in appropriate medium to microplate wells. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. ; The top 3 importers of Spectramax are India with 365. If filters are used with the SpectraMax iD5 reader, integration time may be set to 50 ms, reducing the. Among the various ready-to-read protocols, users will find pre-written SpectraDrop Plate protocols enabling Micro Volume applications measurement. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. Published Dec 16, 2020 | Updated Dec 16, 2020. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. For crystal violet. 2. Page 22 SpectraMax L Luminescence Microplate Reader User Guide To manually change the aperture: 1. These lab workhorses are used in drug discovery, research, bioassay validation, and. The fluorescence intensity of the cells was detected using a SpectraMax M3 florescence microplate reader (Molecular Devices) with excitation and emission wavelengths of 485 and 535 nm, respectively. On the front panel of the instrument, press the load/eject button to move the microplate drawer outside of the instrument. For example, microplates containing different samp les can be read using the same orCell viability는 세포막 Integrity 또는. Select wells and then add or select a group or blank and assignA microplate reader that can detect two or more applications is considered a multi-mode plate reader. 116 SpectraMax M5 and M5e Multi-Mode. Figure 6: Transcreener ADP2 FI assay principle. Microplate Readers Tecan Infinite M200 Plate Reader. Learn how you can utilize microplate readers to drive landmark discoveries. Using a SpectraMax M3 spectrophotometer from Molecular Devices, we measured the color change at 450 nm. SpectraMax DuoLuc Reporter Assay (SMP 6. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. Wavelength precision. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. book Page 21 Wednesday, July 7, 2010 2:09 PM. book Page 1 Wednesday, July 7, 2010 2:09 PM Plate chamber design of the SpectraMax category 1 microplate readers. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. book Page 1 Wednesday, July 7, 2010 2:09 PMThe fluorescent intensity was measured using SpectraMax M3 fluorescence microplate reader (Molecular Devices) every 90 seconds with excitation at 320 nm and emission at 420 nm at 37°C for 2 hours. 7°C (gradient heating design). Microplate Readers include the M3, M4,. The Molecular Devices® SpectraMax® M3 Reader provides three detection modes: UV-Vis absorbance, fluorescence intensity, and luminescence, but can be upgraded to include time-resolved fluorescence and/or fluorescence polarization modes. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part.